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尺寸排阻層析填料 TOYOPEARL HW-75
尺寸排阻層析填料 TOYOPEARL HW-75
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尺寸排阻層析填料 TOYOPEARL HW-75

價(jià)格：面議
品牌：TOYOPEARL 產(chǎn)地：日本 貨期：現(xiàn)貨
AI問答
  • 尺寸排阻層析填料TOYOPEARLHW-75價(jià)格？ 可以試用嗎？
  • 尺寸排阻層析填料TOYOPEARLHW-75參數(shù)規(guī)格？ 配套的儀器設(shè)備？
  • 尺寸排阻層析填料TOYOPEARLHW-75貨期是多久？ 使用注意事項(xiàng)？
參數(shù)規(guī)格
貨號 Description Particle size Housing?material 操作
? 0007469 Toyopearl HW-75F 500 mL 30 - 60 μm 詢底價(jià)
? 0014691 Toyopearl HW-75F 1 L 30 - 60 μm 詢底價(jià)
? 0008072 Toyopearl HW-75F 5 L 30 - 60 μm 詢底價(jià)
? 0007468 Toyopearl HW-75S 500 mL 20 - 40 μm 詢底價(jià)
? 0008071 Toyopearl HW-75S 5 L 20 - 40 μm 詢底價(jià)
產(chǎn)品介紹

Size-exclusion chromatography (SEC), also referred to as gel-filtration chromatography, uses porous particles to separate biological molecules of different sizes. Molecules that are smaller than the pore size can enter the particles and therefore have a longer path length and longer residence time than larger molecules that cannot enter the particles.

Proteins larger than the pore size can not enter the pores and will thus co-elute as the first peak in the chromatogram. These molecules are said to be totally excluded from the pores. Proteins that can at least partially enter the pores will have varying residence times dependent on the formula weight and hydrodynamic radius of each individual protein. Different proteins will therefore have different residence times when passing through the column. Proteins that elute in this portion of a chromatogram are said to be selectively permeable to the pores. Molecules that are smaller than the pore size are able to enter all of the pores in the resin and will therefore have the longest residence times. These molecules typically elute together as the last peak in a chromatogram and are said to be totally permeable to the pores. 

The main application of size exclusion chromatography is the fractionation and purification of synthetic and biological polymers, such as proteins, polysaccharides and nucleic acids. 

The advantages of this method include good separation of large molecules from the small molecules while preserving the biological activity of the particles to be separated. The technique is generally combined with others that further separate molecules by other characteristics, such as pI, charge, and affinity for certain compounds and is typically used as a polishing step.

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